The objective of the present study was to collect different Lactic acid bacterial strains from culture collection centers and screen their functional probiotic characteristics such as acid tolerance, bile tolerance, antibacterial activity and antibiotic sensitivity for their commercial use. Among the six probiotic strains tested, all showed good survivability at high bile salt concentration (0.3 to 2.0 % oxgall) and good growth at a low pH of 1.5 to 3.5. These probiotic species showed good survival abilities in acidic pH of 2.0 to 3.5. Pediococcus acidilactici 252 was able to grow even at a pH of 1.5 also. Among the six lactic acid species, Lactobacillus paraplantarum 321 and Lactobacillus helveticus 194 (except Gentamycin) were resistant to all the antibiotics tested (Co-Trimoxazole, Gentamicin, Norfloxacin and Tetracycline). All these probiotic organisms were screened for their in vitro inhibition ability against pathogenic microorganisms namely, E.coli ATCC, Pseudomonas aeruginosa, Salmonella paratyphi, Staphylococcus aureus. Lactobacillus paraplantarum 321 and Pediococcus acidilactici 252 inhibited the growth of all pathogenic bacteria tested. The study identified Lactobacillus helveticus 194 and Pediococcus acidilactici 252 as potential functional probiotics for future in vivo studies for commercialization in the food industry.
Loading....